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目的 白细胞介素 1 3(IL 1 3)是新近发现的一种抗炎性细胞因子 ,其在肾小球肾炎中的作用尚不清楚 ,该研究探讨脂多糖 (LPS)对体外培养的人肾小球系膜细胞 (HMC)表达IL 1 3作用以及IL 1 3对HMC促炎性细胞因子、趋化因子和促纤维化因子基因表达的影响。方法 体外培养HMC ,加入不同浓度的LPS和 (或 )IL 1 3后 ,用逆转录 -聚合酶链反应和ELISA检测HMCIL 1 3mRNA表达和细胞培养上清液中IL 1 3蛋白含量 ;应用核酸酶保护法检测HMC肿瘤坏死因子 α(TNF α)、白介素 - 1α(IL 1α)、白介素 - 1 β(IL 1 β)、单核细胞趋化蛋白 1(MCP 1 )、白介素 8(IL 8)、转化生长因子 - β1 (TGF β1 )mRNA的表达。 结果 未予LPS刺激的HMC不表达IL 1 3mRNA和蛋白 ;LPS呈剂量依赖性和时间依赖性诱导HMC表达IL 1 3mRNA和分泌IL 1 3蛋白。HMC受LPS刺激后 1 2h即可表达IL 1 3mRNA ,4 8h达高峰 ,72h仍维持在较高的水平。HMC受LPS刺激后 2 4h ,其培养上清液中检测到IL 1 3蛋白 ,4 8h和 72h进一步增加。外源性IL 1 3呈剂量依赖性地抑制LPS诱导的系膜细胞TNF α ,IL 1α ,IL 1 β ,MCP 1 ,IL 8,TGF β1mRNA的表达。应用抗IL 1 3抗体中和内源性IL 1 3后 ,上述炎症因子表达增强。结论 IL 1 3是HMC自分泌因子。IL 1 3可抑制LPS诱导
Objective IL-3 (interleukin-13) is a recently discovered anti-inflammatory cytokine whose role in glomerulonephritis is unclear. This study investigated the effects of lipopolysaccharide (LPS) on human kidney The effect of IL-13 on the expression of mitochondrial mesangial cells (HMCs) and the gene expression of proinflammatory cytokines, chemokines and pro-fibrotic factors on HMCs. Methods HMC was cultured in vitro. After adding different concentrations of LPS and / or IL-13, the expression of HMCIL-13 mRNA and IL-13 protein in cell culture supernatant were detected by reverse transcription-polymerase chain reaction and ELISA. The levels of TNFα, IL-1α, IL-1β, MCP 1, IL-8, Expression of Transforming Growth Factor - β1 (TGFβ1) mRNA. Results HMC without LPS stimulation did not express IL-13 mRNA and protein. LPS induced IL-13 mRNA expression and IL-3 secretion by HMC in a dose-dependent and time-dependent manner. IL-3 mRNA could be expressed 12 h after stimulation with LPS, peaked at 48 h and remained at a high level at 72 h. IL-3 protein was detected in the culture supernatant 24 h after LPS stimulation, and further increased at 48 h and 72 h. Exogenous IL-13 inhibited the LPS-induced TNFα, IL 1α, IL 1β, MCP 1, IL 8 and TGFβ1 mRNA expression in a dose-dependent manner. The above inflammatory cytokine expression is enhanced after neutralization of endogenous IL-13 with anti-IL13 antibody. Conclusion IL-13 is an HMC autocrine factor. IL-13 can inhibit LPS induction