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以555份芒(Miscanthus sinensis)种质资源为研究对象,根据26个表型性状数据,按地理来源、植物区系和单一性状进行分组,分别采用简单比例法、平方根法和多样性指数法确定组内取样数,再根据聚类和随机2种方法进行组内个体选择。依照上述方案共构建出19个具有代表性的芒初选核心种质样本库。通过平均相似系数、性状符合度、数量性状变异系数和遗传多样性指数等4项检测指标对上述19种构建方案进行比较,最终确定了按“植物区划分组+多样性指数确定取样数+聚类选择个体”为芒初级核心种质构建的最佳方案。通过此方法建立起的芒初级核心种质资源共83份,占总资源的14.95%,且新构建的初级种质资源与总资源性状符合度达到100%。
Based on the data of 26 phenotypic traits, 555 Miscanthus sinensis germplasm resources were divided into four groups according to geographical origin, flora and single traits. The results of simple proportion method, square root method and diversity index method The number of samples in the group, and then according to the clustering and random 2 groups of individual selection. A total of 19 representative samples of core collection of mangrove primaries were constructed according to the above scheme. The above 19 kinds of construction schemes were compared by four test indexes, such as average similarity coefficient, trait conformity, quantitative trait coefficient of variation and genetic diversity index, finally determined the number of samples determined according to “plant division group + diversity index + sampling number Class Select Individuals ”Best Practices for Creating Primary Core Collection in Mangosteen. 83 primary mangrove core germplasm resources established by this method accounted for 14.95% of the total resources, and the newly constructed primary germplasm resources matched 100% of the total resource traits.