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用重组人β－珠蛋白基因及其增强子的逆转录病毒载体转染－２细胞及ＰＡ３１７细胞，分离出产病毒粒子的－２和ＰＡ３１７克隆。用这两种细胞克隆进行乒乓上清感染，得到能产生高滴度逆转录病毒载体的ＰＡ３１７细胞克隆，其滴度达５．９×１０~６ＣＦＵ／ｍｌ。用此高滴度的病毒载体，在加入造血生长因子的情况下，重复感染人骨髓单核细胞，Ｓｏｕｔｈｅｒｎ印迹杂交显示人β－珠蛋白基因及其增强子已完整地整合到人骨髓造血细胞基因组上。
-2 cells and PA317 cells were transfected with a retroviral vector containing the recombinant human β-globin gene and its enhancer, and -2 and PA317 clones producing virus particles were isolated. The two cell clones were used for ping-pong supernatant infection to obtain PA317 cell clone capable of producing high-titer retroviral vector with a titer of 5.9 × 10 ~ 6CFU / ml. Using this high titer viral vector, human bone marrow mononuclear cells were repeatedly infected with hematopoietic growth factors and Southern blot hybridization revealed that the human beta-globin gene and its enhancer have been completely integrated into the human bone marrow hematopoietic cell genome on.