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目的 :观察顺铂、5-FU、甲氨蝶呤在体外对人宫颈肿瘤细胞的作用。方法 :用MTT测定法观察以上 3种化疗药物单用及合用对HeLa细胞的影响 ,用流式细胞仪观察凋亡及细胞周期的变化。结果 :顺铂和 5-FU单独应用及合用时 ,随着药物剂量增加抑制效应增加。结论 :顺铂和 5-FU能有效抑制HeLa细胞的生长 ,甲氨蝶呤在本实验条件下无此作用。
Objective: To observe the effect of cisplatin, 5-FU and methotrexate on human cervical cancer cells in vitro. Methods: MTT assay was used to observe the effect of the three chemotherapeutic agents alone and in combination on HeLa cells. The changes of apoptosis and cell cycle were observed by flow cytometry. Results: When cisplatin and 5-FU were used alone or in combination, the inhibitory effect increased with the increase of the dosage of cisplatin and 5-FU. Conclusion: Cisplatin and 5-FU can effectively inhibit the growth of HeLa cells. Methotrexate has no effect in this experiment.