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背景与目的:研究表明,Hedgehog(HH)信号通路为调控胚胎发育过程的重要通路之一,其活化参与多种实体肿瘤的发生,而功能性跨膜蛋白受体Ptched1(PTCH1)等位基因的沉默是HH通路激活和肿瘤发生的关键。本研究旨在探讨PTCH1在人膀胱尿路上皮癌中的表达及其与膀胱癌发生的关系。方法:收集人膀胱尿路上皮癌56例及正常膀胱组织19例,采用Real-time PCR、免疫组化、蛋白质印迹法(Western blot)方法检测标本中PTCH1 mRNA和蛋白的表达情况以及分布规律,分析PTCH1蛋白的表达与膀胱尿路上皮癌的临床生物学特征的关系。结果:PTCH1 mRNA在膀胱尿路上皮癌中的表达明显低于正常膀胱组织(P<0.05)。PTCH1蛋白在膀胱癌中的表达比正常组织低(P<0.01)。PTCH1表达与患者性别和年龄无关(P>0.05)。低分级癌中PTCH1的表达率明显低于高分级癌(P<0.05),侵润性膀胱癌PTCH1的表达率明显低于表浅性膀胱癌(P<0.05)。结论:PTCH1在膀胱尿路上皮癌的表达下调,并可能与膀胱尿路上皮癌的发生、发展密切相关。
BACKGROUND AND PURPOSE: Studies have shown that the Hedgehog (HH) signaling pathway is one of the important pathways regulating embryonic development. Its activation is involved in the development of many solid tumors. The function of the Ptched1 (PTCH1) allele Silencing is the key to HH pathway activation and tumorigenesis. This study aimed to investigate the expression of PTCH1 in human bladder urothelial carcinoma and its relationship with the occurrence of bladder cancer. Methods: Fifty-six human urothelial carcinoma tissues and 19 normal bladder tissues were collected. The expression and distribution of PTCH1 mRNA and protein were detected by Real-time PCR, immunohistochemistry and Western blot. The relationship between the expression of PTCH1 protein and the clinical biological characteristics of bladder urothelial carcinoma was analyzed. Results: The expression of PTCH1 mRNA in bladder urothelial carcinoma was significantly lower than that in normal bladder tissue (P <0.05). The expression of PTCH1 protein in bladder cancer was lower than that in normal tissues (P <0.01). The expression of PTCH1 was not related to the gender and age of the patients (P> 0.05). The expression of PTCH1 in low-grade cancer was significantly lower than that in high-grade cancer (P <0.05). The expression of PTCH1 in invasive bladder cancer was significantly lower than that in superficial bladder cancer (P <0.05). Conclusion: The expression of PTCH1 is down-regulated in bladder urothelial carcinoma and may be closely related to the occurrence and development of bladder urothelial carcinoma.