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采用反相高效液相色谱法 (以柔红霉素为内标 )测定血浆中表阿霉素的含量 ,流动相为 5mmol/LH3PO4-甲醇 -异丙醇 -乙晴 (6∶9∶7∶2 ,pH2 .9) ,YWGC18不锈钢色谱柱 ,荧光检测波长 :λex=4 5 0nm ,λem=5 30nm。血浆中样品经氯仿 -甲醇 -异丙醇 =5∶2∶3抽提液抽提处理后进样测定。血浆中表阿霉素的浓度在 0 .0 3～2 4mg/L范围内峰面积与柔红霉素的峰面积之比呈线性关系 (r =0 .9998) ,方法对表阿霉素的平均回收率为89 %～ 90 .7%。最低检测浓度为 0 .0 1mg/L。本法灵敏度高 ,线性范围大 ,符合临床用药的血药浓度监测和药代动力学研究的分析方法要求 ,并用于 4 0例已服用表阿霉素的癌症病人的血浆药物含量的测定。
The content of epirubicin in plasma was determined by RP-HPLC with daunorubicin as internal standard. The mobile phase consisted of 5mmol / LH3PO4-methanol-isopropanol-acetonitrile (6: 9: 7: 2, pH2 .9), YWGC18 stainless steel column, fluorescence detection wavelength: λex = 450nm, λem = 530nm. Plasma samples were extracted by chloroform - methanol - isopropanol = 5: 2: 3 extraction solution after injection determination. The concentration of epirubicin in plasma in the range of 0.3 ~ 24mg / L peak area and daunorubicin peak area ratio was linear (r = 0.9998), the method of epirubicin The average recovery was 89% ~ 90 .7%. The lowest detection concentration is 0 .0 1mg / L. This method has high sensitivity, large linear range, in line with clinical drug concentration monitoring and pharmacokinetic analysis of analytical methods, and used in 40 cases of patients already taking epirubicin in the determination of plasma drug levels.