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目的:观察更年春方对去卵巢(OVX)大鼠骨髓微环境中骨保护素(OPG)、破骨细胞分化因子(RANKL)和巨噬细胞集落刺激因子(M-CSF)mRNA表达的影响,探讨更年春抗绝经后骨质疏松骨丢失的可能分子机制。方法:取健康10月龄雌性SD大鼠42只,随机平均分为假手术组(S),生理盐水组(N)、尼尔雌醇组(E)、更年春高剂量组(H)、更年春中剂量组(M)和更年春低剂量组(L)。假手术组仅切除卵巢周围少许脂肪,其余5组均切除卵巢。灌药3d后取腰椎冲洗骨髓提取总RNA,实时定量PCR法检测上述因子mRNA的表达情况。结果:更年春高剂量组可上调OVX大鼠骨组织中OPG的mRNA的表达,下调M-CSF的mRNA的表达,使RANKL/OPG值下降。结论:更年春方抗绝经后骨质疏松骨丢失的分子机制可能与其调控OPG和M-CSF的表达,影响RANKL/OPG值有关。
OBJECTIVE: To observe the effect of Geng Ninchun Prescription on osteoprotegerin (OPG), osteoclast differentiation factor (RANKL) and macrophage colony-stimulating factor (M-CSF) mRNA expression in the bone marrow microenvironment of ovariectomized (OVX) rats. To explore the possible molecular mechanism of postmenopausal osteoporosis bone loss after the new year spring. Methods: A total of 42 healthy 10-month-old female Sprague-Dawley rats were randomly divided into sham operation group (S), saline group (N), nilestriol group (E), and Gannengchun high-dose group (H). The Zhongnengchun middle-dose group (M) and the Zhongnianchun low-dose group (L). In the sham operation group, only a small amount of fat around the ovary was removed, and the rest of the 5 groups were ovariectomized. The total RNA was extracted from the bone marrow of the lumbar vertebrae 3 days after the drug injection, and the mRNA expression of the above factors was detected by real-time quantitative PCR. RESULTS: The high-dose group of Gengnianchun could up-regulate the mRNA expression of OPG in OVX rats, down-regulate the mRNA expression of M-CSF, and decrease the RANKL/OPG value. Conclusion: The molecular mechanism of postmenopausal osteoporosis bone loss after Gengnianchun treatment may be related to its regulation of OPG and M-CSF expression and affecting RANKL/OPG values.