Background The commonly used method so far for formulating hyperlipidemia animal models is to feed the animals with a high fat diet. Owning to influences of animal feeding, dosage and cycle, feeding with a high fat diet has resulted in an unstable quality. It is unknown whether gastric perfusion of intralipid can establish a better hyperlipidemia model in rabbits. Methods Twenty rabbits were randomly selected and subjected to gastric intralipid perfusion for consecutive 2 weeks or to feed with common high cholesterol feed for 4 weeks. The levels of blood serum total cholesterol(TC), triglyceride(TG), apolipoprotein A1(APOA1), APOB, blood plasma thromboxane B2(TXB2), 6-Keto-prostaglandin F1α(6-Keto-PGF1α),endotheline(ET), erythrocyte membrane cholesterol(M-Tc), membrane fluidity(M-Flu), erythrocyte malondialdehyde(E-MDA), erythrocyte superoxide dismutase(E-SOD) before and after the experiment. In the end of the experiment, the aortae were removed from all the animals for light and electron microscopic examinations. Results The results showed that in both groups, the levels of blood serum TC, TG, APOB,blood plasma TXB2, ET, erythrocyte M-Flu and E-MDA were significantly increased. Blood serum APOA1,blood plasma 6-Keto-PGF1α and E-SOD were significantly decreased. But, erythrocyte M-TC had no significantly changed. The indexes of the two groups were no obvious difference before and after the experiment. Light and electron microscopic examination showed that there were early changes of early atherosclosis in the two groups of animal, in accordance with the characteristics of experimental animal hyperlipidemia. Conclusion The results suggest that gastric perfusion of intralipid can produce hyperlipidemia in rabbits faster than the commonly used model developed by feeding high cholesterol diet. Background The commonly used method so far for formulating hyperlipidemia animal models is to feed the animals with a high fat diet. Owning to influences of animal feeding, dosage and cycle, feeding with a high fat diet has resulted in an unstable quality. It is unknown whether gastric perfusion of intralipid can establish a better hyperlipidemia model in rabbits. Methods Twenty rabbits were randomly selected and subjected to gastric intralipid perfusion for 2 weeks or to feed with common high cholesterol feed for 4 weeks. The levels of blood serum total cholesterol TC, triglyceride (TG), apolipoprotein A1 (APOA1), APOB, blood plasma thromboxane B2 (TXB2), 6-Keto- prostaglandin F1α (6-Keto-PGF1α), endotheline (ET), erythrocyte membrane cholesterol In the end of the experiment, the aortae were removed from all the animals for light and (E-SOD) before and after the experiment. ele Results showed that both levels of blood serum TC, TG, APOB, blood plasma TXB2, ET, erythrocyte M-Flu and E-MDA were significantly increased. Blood serum APOA1, blood plasma 6- But, erythrocyte M-TC had no significantly changed. The indexes of the two groups were no obvious difference before and after the experiment. Light and electron microscopic examination showed there were early changes of early atherosclosis in the two groups of animal, in accordance with the characteristics of the experimental of animal hyperlipidemia. Conclusion The results suggest that gastric perfusion of intralipid can produce hyperlipidemia in rabbits faster than the commonly used model model developed by feeding high cholesterol diet.