The copper recovery from low-grade copper sulfide ore was investigated using microbial leaching. Several parameters substantially affect the bioleaching of copper; among them, pulp density and nutrient media were selected for investigation. The optimum conditions for copper recovery were a pulp density of 5 g/mL, a mixed-mineral salt medium of Acidithiobacillus thiooxidans(70vol%) and Acidithiobacillus ferrooxidans(30vol%), and 10vol% of inoculum. Under these conditions, the maximum bioleaching capacity of the medium for copper recovery was determined to be approximately 99%. The effect of pulp density on the kinetics of the bioleaching process was surveyed using both da Silva’s method and constrained multilinear regression analysis. The kinetics of copper dissolution followed the shrinking core model, and the process was diffusion controlled at a pulp density of 5 g/mL. Nevertheless, at higher pulp densities, the process was controlled by chemical reaction. The copper recovery from low-grade copper sulfide ore was investigated using microbial leaching. Several optimum substantially the bioleaching of copper; among them, pulp density and nutrient media were selected for investigation. g / mL, a mixed-mineral salt medium of Acidithiobacillus thiooxidans (70 vol%) and Acidithiobacillus ferrooxidans (30 vol%), and 10 vol% of inoculum. 99%. The effect of pulp density on the kinetics of the bioleaching process was surveyed using both da Silva’s method and constrained multilinear regression analysis. The kinetics of copper dissolution followed the shrinking core model, and the process was diffusion controlled at a pulp density of Nevertheless, at higher pulp densities, the process was controlled by chemical reaction.