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目的:建立一种用毛细管电泳拆分盐酸安非他酮对映异构体的色谱方法。方法:以60 cm×75μm石英毛细管柱为分离通道,通过考察影响拆分的因素,如手性选择剂的种类、浓度,缓冲溶液的pH,分离电压和柱温,确定最佳拆分条件。结果:安非他酮拆分的最优条件为浓度15 mmol·L-1羟丙基-β-环糊精作手性添加剂,50 mmol·L-1的磷酸盐(pH 3.5)背景缓冲液,工作电压20 kV,柱温25℃。确定毛细管电泳中先出峰的是S-盐酸安非他酮。结论:该方法试样用量少、高效、快速、操作简单,环境污染少,可用于盐酸安非他酮的拆分,并为盐酸安非他酮的含量测定和单一对映体药理作用的研究奠定基础。
OBJECTIVE: To establish a chromatographic method for the separation of bupropion hydrochloride enantiomers by capillary electrophoresis. Methods: A 60 cm × 75 μm quartz capillary column was used as the separation channel. The optimal resolution conditions were determined by investigating factors affecting the resolution, such as the type and concentration of chiral selector, pH of buffer solution, separation voltage and column temperature. Results: The optimum conditions for the separation of bupropion were 15 mmol·L -1 hydroxypropyl-β-cyclodextrin as chiral additive, 50 mmol·L -1 phosphate (pH 3.5) as background buffer, Operating voltage 20 kV, column temperature 25 ℃. Determining the first peak in capillary electrophoresis is S-HCl bupropion. Conclusion: This method has the advantages of less amount of sample, high efficiency, rapidness, simple operation, less environmental pollution, resolution of bupropion hydrochloride and determination of bupropion hydrochloride and single enantiomer Research laid the foundation.