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衣原体是一类专性在真核细胞内营寄生生活的微生物 ,可在动物和人中引起多种疾病 ,近年来越来越引起人们的重视 ,对衣原体的研究也逐步深入。传统的衣原体分类鉴定方法包括培养特性、形态学观察、血清学试验和致病性等。引入遗传学方法后 ,特别是近年来将聚合酶链式反应和限制性内切酶片段长度多态性分析技术应用到衣原体分类鉴定中 ,使衣原体分类学得到了进一步发展。通常用来扩增的目的基因是衣原体主要外膜蛋白基因和16S～ 2 3SRNA基因间隔区及其侧翼序列 ,以及omp2及 2 3SRNA区域 1等。
Chlamydia is a kind of microorganism that specializes in living parasitism in eukaryotic cells. It can cause many diseases in animals and humans and has drawn more and more attention in recent years. The research of Chlamydia is also gradually deepened. Traditional Chlamydia classification identification methods include culture characteristics, morphological observation, serological tests and pathogenicity. The introduction of genetic methods, especially in recent years, polymerase chain reaction and restriction fragment length polymorphism analysis technology applied to the classification of Chlamydia identification, Chlamydia taxonomy has been further developed. Commonly used to amplify the target gene is Chlamydia major outer membrane protein genes and 16S ~ 23SRNA gene spacer region and its flanking sequences, and omp2 and 23SRNA region 1 and so on.