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目的:通过动物实验探讨啤酒酵母胞壁多糖对肿瘤的抑制作用。方法:采用现代分离提取技术从啤酒废酵母中分离纯化出酵母胞壁多糖;通过小鼠移植性肿瘤试验探讨酵母胞壁多糖在体内的抑瘤作用,并对其进行半致死量测定。结果:酵母胞壁多糖能显著抑制HCa-F25/A16肝癌和Lewis肺癌的瘤重(在剂量为0～200mg/kg·d范围内抑制率随剂量增加而增加,当剂量超过200mg/kg·d时,抑制率反而下降);该多糖小鼠口服时的半致死量LD_(50)为3077.86(mg/kg)。结论:使用本工艺获得的样品,其酵母胞壁多糖的含量为86.5％;酵母胞壁多糖对Hca-F25/A16肝癌和Lewis肺癌具有显著的抑制作用;急性毒理实验说明酵母胞壁多糖是一种安全低毒、值得开发的保健食品的原料资源。
Objective: To investigate the inhibitory effect of Saccharomyces cerevisiae cell wall polysaccharide on tumor by animal experiments. Methods: Separation and purification of yeast cell wall polysaccharides from waste beer yeast by modern separation and extraction technology. To study the antitumor activity of the cell wall polysaccharide of yeast in vivo by transplanted tumor in mice and to determine its semi-lethality. Results: Saccharomyces cerevisiae polysaccharide could significantly inhibit the tumor weight of HCa-F25 / A16 and Lewis lung cancer (the inhibition rate increased with the increase of dose in the range of 0 ~ 200mg / kg · d. When the dosage exceeded 200mg / kg · d The inhibition rate decreased). The LD50 of the polysaccharide in mice was 3077.86 (mg / kg). Conclusion: The sample obtained by this process has a yeast cell wall polysaccharide content of 86.5%, and the yeast cell wall polysaccharide has a significant inhibitory effect on Hca-F25 / A16 liver cancer and Lewis lung cancer. The acute toxicity experiment indicates that the yeast cell wall polysaccharide is A safe and low toxicity, worth developing raw material resources of health food.