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目的探讨REGIα基因在原发性胃癌组织中的表达状况与胃癌临床病理特性和患者生存率之间的关系。方法采用RT-PCR及免疫组织化学方法检测胃癌组织REGIα基因表达水平,并统计分析它们与胃癌临床病理特征的相关性。结果用RT-PCR及免疫组织化学法筛查235例原发性胃癌患者后,其结果显示:有78%(183/235)的原发性胃癌REG IαmRNA阳性,而REG Iα蛋白呈阳性反应只有73例,占31.1%(73/235),其中浸润性生长的肿瘤REG Iα蛋白表达率远高于局限性生长(P<0.05)。REGIαmRNA及蛋白在胃癌中的表达与肿瘤的浸润生长方式、印戒细胞癌及低分化胃腺癌关系密切。REGIαmRNA及蛋白阳性的高分化胃腺癌发生血行转移的机会显著高于其阴性表达的肿瘤。与REGIαmRNA及蛋白表达阳性的肿瘤患者相比,REGIαmRNA及蛋白表达阴性的高分化腺癌患者有更好的预后。结论REGIαmRNA及蛋白的表达与胃癌细胞的分化状态,细胞浸润性生长方式和印戒细胞癌密切相关,并可大大增强高分化腺癌血行转移的风险,可视做高分化胃腺癌的一个不良预后因子。
Objective To investigate the relationship between the expression of REGIα gene in primary gastric cancer and the clinicopathological features and survival rate of patients with gastric cancer. Methods The expression of REGIα gene in gastric cancer tissues was detected by RT-PCR and immunohistochemistry, and their correlations with clinicopathological characteristics of gastric cancer were statistically analyzed. Results After screening 235 patients with primary gastric cancer by RT-PCR and immunohistochemistry, the results showed that REG Iα mRNA was positive in 78% (183/235) of primary gastric cancers and positive in REG Iα protein 73 cases, accounting for 31.1% (73/235). The expression of REG Iα protein in infiltrative tumors was much higher than that in limited growth (P <0.05). The expression of REGIαmRNA and protein in gastric cancer is closely related to the invasive growth mode, signet ring cell carcinoma and poorly differentiated gastric adenocarcinoma. The chances of hematogenous metastasis of REGIα mRNA and protein-positive well-differentiated gastric adenocarcinoma were significantly higher than those of negative-expressing tumors. Patients with well-differentiated adenocarcinoma of REGIα mRNA and protein expression had a better prognosis than those with positive REGIα mRNA and protein expression. Conclusions The expression of REGIα mRNA and protein is closely related to the differentiation status, cell infiltrative growth pattern and signet ring cell carcinoma of gastric cancer cells, and can greatly enhance the risk of hematogenous metastasis in well-differentiated adenocarcinoma. It can be regarded as an unfavorable prognosis of well-differentiated gastric adenocarcinoma factor.