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目的研究龙葵多糖对S180及H22荷瘤小鼠红细胞补体受体1(CR1)数量及活性的影响,探讨龙葵多糖抗肿瘤作用的红细胞免疫机制。方法采用体内移植S180和H22肿瘤细胞株造模的方法,评价龙葵多糖的体内抗肿瘤疗效;红细胞免疫花环实验检测红细胞免疫黏附肿瘤细胞的能力;CR1单克隆抗体标记,流式细胞术检测荷瘤小鼠红细胞CR1数量;酵母多糖免疫黏附荷瘤小鼠红细胞的能力评价荷瘤小鼠红细胞CR1受体的活性;比色法测定荷瘤小鼠红细胞膜唾液酸含量。结果龙葵多糖能够抑制S180荷瘤小鼠实体瘤生长,延长H22荷瘤小鼠生存时间;提高荷瘤小鼠红细胞免疫花环率(DTER);增加荷瘤小鼠红细胞CR1受体的数量和活性;增加荷瘤小鼠红细胞膜唾液酸含量。结论龙葵多糖通过提高荷瘤小鼠红细胞膜上唾液酸含量,进而提高CR1的活性和数量,改善和提高荷瘤小鼠红细胞免疫黏附能力,发挥抗肿瘤的作用。
Objective To investigate the effect of polysaccharides of Solanum nigrum on the number and activity of erythrocyte complement receptor 1 (CR1) in S180 and H22 tumor-bearing mice, and to explore the anti-tumor effect of solanum polysaccharide on erythrocyte immune mechanism. Methods The in vivo antitumor effects of polysaccharides from Solanum nigrum L. were evaluated by the methods of in vivo transplantation of S180 and H22 tumor cell lines. The erythrocyte immune rosette assay was used to detect the ability of erythrocytes to adhere to tumor cells. The CR1 monoclonal antibody labeled and flow cytometry The number of erythrocytes CR1 in tumor-bearing mice; the ability of zymosan to adhere erythrocytes of tumor-bearing mice to evaluate the activity of erythrocyte CR1 receptor in tumor-bearing mice; and the content of sialic acid in erythrocytes of tumor-bearing mice by colorimetric assay. Results Solanum nigrum polysaccharide could inhibit the growth of solid tumor in S180 tumor-bearing mice, prolong the survival time of H22 tumor-bearing mice, increase the rate of erythrocyte immune rosette (DTER) in tumor-bearing mice and increase the number and activity of erythrocyte CR1 receptor in tumor-bearing mice ; Increase the erythrocyte membrane sialic acid content in tumor-bearing mice. Conclusion Solanum nigrum polysaccharide can improve the activity and quantity of CR1, improve and enhance the erythrocyte immune adhesive ability of tumor-bearing mice, and exert its antitumor effect by increasing the sialic acid content of the erythrocyte membrane of tumor-bearing mice.