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目的研究慢性乙醇中毒引起小鼠脑神经细胞Ⅰ型1,4,5-三磷酸肌醇受体(IP3R1)表达的变化。方法 40只小鼠随机分为90d、180d组,各组再分为正常对照组、10%、20%、30%乙醇组,每组5只,乙醇组给予相应浓度乙醇饮用至相应时间;取各组小鼠脑组织,分别采用免疫组化和Western blot方法检测脑皮质神经细胞IP3R1表达的变化;SPSS 13.0软件对实验数据进行单因素方差分析。结果正常IP3R1免疫组化染色物分布于神经细胞胞浆内。90d组随乙醇浓度的增加,IP3R1免疫组化阳性细胞数和阳性细胞率逐步增加,组间比较,差异显著(P<0.05);180d组中10%、20%乙醇组阳性细胞数和阳性细胞率逐步增加,组间比较,差异显著(P<0.05),而30%乙醇组阳性细胞数和阳性细胞率反而减少,且低于90d组的相同浓度组(P<0.05)。Western blot与免疫组化检测结果基本一致。结论慢性乙醇中毒可引起小鼠大脑皮质神经细胞IP3R1表达增加,而高浓度(30%)、长时间(180d)乙醇使IP3R1表达降低,可能与神经细胞变性、坏死、数目减少有关。
Objective To investigate the changes of type I 1,4,5-triphosphate inositol 1,4-inositol 1,4,5-trisphosphate receptor (IP3R1) induced by chronic alcoholism in mice. Methods Forty mice were randomly divided into 90 d and 180 d groups. Each group was divided into normal control group, 10%, 20% and 30% ethanol group, with 5 rats in each group. The changes of IP3R1 expression in cerebral cortex neurons were detected by immunohistochemistry and Western blot, respectively. The single-factor analysis of variance (ANOVA) was used to analyze the experimental data by SPSS 13.0 software. Results Normal IP3R1 immunohistochemical staining distribution in neuronal cytoplasm. The number of IP3R1 immunohistochemical positive cells and the percentage of positive cells in 90d group increased gradually with the increase of ethanol concentration, and there was significant difference between the two groups (P <0.05). The number of positive cells and positive cells in 10%, 20% (P <0.05). However, the number of positive cells and the percentage of positive cells in the 30% ethanol group decreased but were lower than those in the 90d group (P <0.05). Western blot and immunohistochemistry results are basically the same. Conclusion Chronic alcoholism can induce IP3R1 expression in cerebral cortex of mice. However, IP3R1 expression is decreased in high concentration (30%) and long-term (180d) ethanol, which may be related to degeneration, necrosis and number of neurons.