最近对治疗用蛋白质的研究包括组织纤维溶酶激活剂,红细胞生成素和粒细胞集落刺激因子,这些研究表明与它们相连的糖链在许多生物学和物理学性质上起着重要的作用。细胞集落刺激因子(G-CSF)和尿激酶原(proUK)的研究表明,对它们有目的地引进N型寡糖链能改进在体内和体外的生物学活性,如对蛋白酶的敏感性,热稳定性和在体内半衰期的延长。 Recent studies on therapeutic proteins include tissue plasminogen activator, erythropoietin, and granulocyte colony-stimulating factor. These studies indicate that the sugar chains associated with them play an important role in many biological and physical properties. Studies of G-CSF and proUK have shown that targeted introduction of N-type oligosaccharide chains to them improves biological activity both in vitro and in vivo, such as sensitivity to proteases, heat Stability and in vivo half-life extension.