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目的：研究Ｎａ＋／Ｈ＋交换泵－１（ＮＨＥ－１）在肺癌细胞增殖／凋亡调控中的作用。方法：采用荧光探针法检则ＮＨＥ－１抑制剂双甲基氨氯吡咪（ＤＭＡ）对人肺巨细胞癌高转移株（ＰＬＡ－８０１Ｄ）细胞的酸化作用；以活细胞计数法检测ＤＭＡ对ＰＬＡ－８０１Ｄ细胞增殖生长的抑制作用；采用原位细胞凋亡检测法观察ＤＭＡ诱导ＰＬＡ－８０１Ｄ细胞的凋亡。结果：经２５μｍｏｌ／Ｌ以上ＤＭＡ处理２４ｈ或５０μｍｏｌ／Ｌ处理８ｈ以上即能酸化ＰＬＡ－８０１Ｄ细胞，其细胞内ｐＨ（ｐＨｉ）显著降低，并诱导了ＰＬＡ－８０１Ｄ细胞的凋亡，肿瘤细胞的增殖生长也显著受抑。结论：ＮＨＥ－１在肺癌细胞增殖／凋亡调控中起重要作用，抑制其增强的活性可能具有一定的应用前景
Objective: To study the role of Na+/H+ exchange pump-1 (NHE-1) in the regulation of proliferation/apoptosis of lung cancer cells. METHODS: Fluorescence probe was used to detect the acidification of human lung giant cell carcinoma high metastasis (PLA-801D) cells by the NHE-1 inhibitor dimethylaminopyrrolide (DMA); DMA was measured by viable cell counting method. Inhibition of proliferation and growth of PLA-801D cells; In situ apoptosis assay was used to observe the apoptosis of PLA-801D cells induced by DMA. RESULTS: PLA-801D cells could be acidified after treated with DMA for 24 h or 50 μmol/L for more than 8 h at 25 μmol/L, and the intracellular pH (pHi) was significantly reduced, and apoptosis of PLA-801D cells was induced, and the proliferation of tumor cells was induced. Growth is also significantly suppressed. Conclusion: NHE-1 plays an important role in the regulation of proliferation/apoptosis of lung cancer cells, and inhibition of its enhanced activity may have certain application prospects.