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目的制备及评价一种用于血管内栓塞治疗肝癌的新型栓塞材料原位植烷三醇液晶。方法通过三元图实验设计筛选原位植烷三醇液晶的最佳处方,测定其黏度及胶凝时间,并考察此原位植烷三醇液晶的生物相容性;采用数字减影血管造影术检查评价原位植烷三醇液晶的栓塞效果,术后1周取栓塞组织石蜡切片,常规HE染色,光镜观察。结果当水的质量分数小于50%时,所得各向同性溶液具有良好的流动性,并能在体内迅速形成植烷三醇立方液晶;另外,植烷三醇液晶无细胞毒性,不引起溶血,对出血和凝血时间无影响;栓塞实验表明该植烷三醇液晶能有效地闭塞血管,阻断肝动脉的血流,病理检查发现栓塞7 d后植烷三醇液晶仍能滞留于血管中。结论植烷三醇液晶具有良好的生物相容性及栓塞效果,是一种理想的新型栓塞材料。
Objective To prepare and evaluate a novel embolic trimer liquid crystal for embolization in the treatment of liver cancer. Methods The optimum prescription of phytantriol liquid crystal in situ was screened through ternary diagram design, and the viscosity and gelation time were measured. The biocompatibility of this in situ phytantriol liquid crystal was investigated. The digital subtraction angiography Intraoperative evaluation of embolization in situ phytantriol liquid embolization effect, embolization tissue paraffin section 1 week after surgery, conventional HE staining, light microscopy. Results When the mass fraction of water is less than 50%, the resulting isotropic solution has good fluidity and can rapidly form phytantriol cubic liquid crystal in the body. In addition, the phytantriol liquid crystal has no cytotoxicity and does not cause hemolysis, No effect on bleeding and clotting time; embolization experiments showed that phytantriol liquid crystal can effectively occlude the blood vessels, blocking the hepatic artery blood flow, pathological examination found that 7 days after embolization of phytantriol liquid crystals can remain in the blood vessels. Conclusion Phytantriol liquid crystal has a good biocompatibility and embolization effect, is an ideal new embolic material.