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目的:建立测定归脾丸中远志皂苷元和远志酸含量的高效毛细管电泳法。方法:采用未涂层毛细管柱(75μm×57 cm,有效长度50 cm),运行缓冲液为20 mmol.L-1硼砂溶液与20%甲醇(pH=8.0),分离电压25 kV,检测波长210 nm。结果:远志皂苷元、远志酸的线性范围分别为13.25～212μg.mL-1(r=0.9971)和12.5～200μg.mL-1(r=0.9977),平均回收率(n=5)分别为101.5%和100.4%。结论:本法简便、准确,重复性好,可用于归脾丸的质量控制。
OBJECTIVE: To establish a high performance capillary electrophoresis method for the determination of Zuipiwan Zhongyuan sapogenin and polygala root acid. Methods: Uncoated capillary column (75 μm × 57 cm, effective length 50 cm) was used. The running buffer was 20 mmol·L-1 borax solution and 20% methanol (pH = 8.0). The separation voltage was 25 kV and the detection wavelength was 210 nm. Results: The linear ranges of tectorigenin and polygala are 13.25 ~ 212μg.mL-1 (r = 0.9971) and 12.5 ~ 200μg.mL-1 (r = 0.9977) .The average recoveries (n = 5) % And 100.4%. Conclusion: This method is simple, accurate and reproducible. It can be used for the quality control of Guipi Pills.