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目的利用代谢组学方法探究保健药黄精对机体的作用机制.方法将超高效液相色谱-质谱联用技术(UPLCMS)与主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)相结合,对大鼠以黄精灌胃后体内内源性物质的变化情况进行研究.结果在PCA与OPLS-DA模型中,正常组与给药组大鼠血液样本均可明显区分.大鼠给药后共获得6个生物标记物,分别α-生育三烯酚、13’-羧基-γ-生育酚、硬脂酸、胆固醇、溶血磷脂酰乙醇胺[0:0/18:2(9Z,12Z)]、溶血磷脂酰乙醇胺[0:0/20:4(8Z,11Z,14Z,17Z)],其中α-生育三烯酚、13’-羧基-γ-生育酚、硬脂酸的含量升高,而胆固醇、溶血磷脂酰乙醇胺[0:0/18:2(9Z,12Z)]、溶血磷脂酰乙醇胺[0:0/20:4(8Z,11Z,14Z,17Z)]的含量降低.结论蒙药玉竹具有保健作用的原因可能是由于其改变了血液中某些成分的含量。
OBJECTIVE: To study the mechanism of action of huangmin on human body by using metabonomics.METHODS, Orthogonal Partial Least Squares Discriminant Analysis (OPLS- DA) to study the changes of endogenous substances in rats after intragastric administration of Polygonatum.Results In PCA and OPLS-DA model, the blood samples of rats in normal group and administration group can be distinguished obviously After administration, 6 biomarkers were obtained, which were α-tocotrienol, 13’-carboxy-γ-tocopherol, stearic acid, cholesterol and lysophosphatidylethanolamine [0: 0/18: 2 , 12Z)], lysophosphatidylethanolamine [0: 0/20: 4 (8Z, 11Z, 14Z, 17Z)] in which alpha-tocotrienol, 13’-carboxy- gamma- tocopherol, The content of cholesterol, lysophosphatidylethanolamine [0: 0/18: 2 (9Z, 12Z)], lysophosphatidylethanolamine [0: 0/20: 4 (8Z, 11Z, 14Z, 17Z) Lower.Conclusion Mongolian medicine Polygonatum has the health effects may be due to changes in the blood of some of the ingredients.