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目的观察肌萎灵冻干粉对大鼠脊髓损伤后的神经保护作用,并研究其对谷氨酸诱导的原代神经细胞凋亡的影响。方法以质量30 g静态压迫硬膜外脊髓10 min建立大鼠脊髓损伤模型,于术后第1天、7天、14天进行各实验组神经功能评价,并观察损伤处脊髓形态学改变;建立低浓度谷氨酸诱导原代神经细胞的兴奋性毒性损伤模型,MTT法检测细胞活力,流式细胞仪检测细胞凋亡率,Western检测Bcl-2、Bax蛋白表达变化。结果 (1)肌萎灵冻干粉可明显提高脊髓损伤大鼠各时间段的BBB评分,改善后肢运动功能,减轻脊髓神经元水肿。(2)肌萎灵冻干粉可增强谷氨酸损伤后的神经细胞活力,缓解神经细胞损伤。(3)肌萎灵冻干粉能显著减少谷氨酸诱导的神经细胞凋亡。(4)肌萎灵冻干粉可不同程度的上调Bcl-2蛋白表达,下调Bax蛋白表达。结论肌萎灵冻干粉可促进脊髓损伤大鼠后肢运动功能恢复,增强神经细胞活力,抑制谷氨酸诱导的神经细胞凋亡,调节凋亡相关蛋白表达,具有一定的神经保护作用。
Aim To observe the neuroprotective effects of freeze-dried muscle wang ling on spinal cord injury in rats and its effect on the apoptosis of primary cultured neurons induced by glutamate. Methods The model of spinal cord injury was established by static compression of the epidural spinal cord at a mass of 30 g for 10 min. The neurological function of each experimental group was evaluated on the 1st, 7th and 14th days after surgery. Morphological changes of the spinal cord were observed. Low concentration of glutamate induced excitotoxic injury model of primary neurons. Cell viability was measured by MTT assay. Apoptosis rate was detected by flow cytometry. Western blot was used to detect the protein expression of Bcl-2 and Bax. Results (1) Lymphagnera lyophilized powder can significantly improve the BBB score of rats with spinal cord injury at different time points, improve motor function of hind limbs and relieve spinal cord neuron edema. (2) Muscle spirits freeze-dried powder can enhance the vitality of nerve cells after glutamate injury, relieve nerve cell injury. (3) Lymphatic freeze-dried powder can significantly reduce glutamate-induced neuronal apoptosis. (4) Muscle spargan lyophilized powder can up-regulate the expression of Bcl-2 protein and down-regulate Bax protein to varying degrees. Conclusion Freeze-dried muscle powder can promote the recovery of motor function of hind limbs in spinal cord injured rats, enhance the activity of nerve cells, inhibit the apoptosis of neurons induced by glutamate, and regulate the expression of apoptosis-related proteins, which has some neuroprotective effects.