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水稻花药培养工作中首先碰到的问题是培养物的污染,常导致预定试验计划的失败。特别在高温多湿的南方稻区,污染是普遍存在的严重问题。接种花药时的污染可用严格消毒、严格无菌操作加以防止,但培养过程中的污染却更难于防止。试管培养到20天后,就往往出现菌丝透过棉塞侵入管中,使培养基污染而报废,这种现象叫做穿透污染。即使反复经过(1.5公斤/cm~2)2小时消毒的棉塞,外面再包一层无菌牛皮纸,在
The first problem encountered in rice anther culture is the contamination of the culture, which often leads to the failure of a scheduled test program. Especially in hot and humid southern rice areas, pollution is a widespread problem. Inoculation of anther contamination can be strictly sterilized, strictly aseptic operation to prevent, but the cultivation process of the pollution is more difficult to prevent. After 20 days of test tube culture, mycelium often invades into the tube through the tampon, so that the medium is contaminated and discarded. This phenomenon is called through-contamination. Even after repeated (1.5 kg / cm ~ 2) 2 hours sterilized tampon, and then covered with a layer of sterile kraft paper, in the