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用20年生闽楠基部萌芽枝条为外植体,从接种时期、取材部位、愈伤组织诱导、增殖和分化、生根培养方面,探讨闽楠的离体快繁技术。结果表明:在春季接种,诱导率高而褐化率低,分别为95.8%和6.5%;茎段诱导率89%,愈伤组织呈白色,易分化,为最优部位;BA与NAA对愈伤组织诱导影响最大,最佳诱导培养基为:MS+6-BA2.0mg/L+NAA0.5mg/L,诱导率为100%。最优分化和增殖(系数4.4)培养基是BA2.0+NAA0.1mg/L,用1/2MS+IBA0.5mg/L的培养基,生根率可达98%。
With 20-year-old basil buds as explants, the in vitro propagation of Min Nan was explored in terms of inoculation period, culture site, callus induction, proliferation and differentiation and rooting. The results showed that inoculation in spring, the induction rate was high and browning rate was low, which was 95.8% and 6.5% respectively; the induction rate of stem section was 89%, the callus was white and easy to differentiate, which was the optimal site; The best inducing medium was MS + 6-BA 2.0 mg / L + NAA 0.5 mg / L, and the induction rate was 100%. Optimal differentiation and proliferation (coefficient of 4.4) medium is BA2.0 + NAA0.1mg / L, with 1 / 2MS + IBA0.5mg / L medium, the rooting rate of up to 98%.