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转化了乙肝病毒 preS2 S基因的重组巴斯德毕赤酵母菌株经甘油培养基充分增殖 ,然后转移到甲醇培养基中进行诱导表达。破碎细胞并提取胞内蛋白 ,经ELISA和WesternBlot检测 ,证明有 4株GS1 1 5 /HIS+MUT+表达了HBVM蛋白。
The recombinant Pichia pastoris strain transformed with hepatitis B virus preS2 S gene was fully propagated in glycerol medium and then transferred to methanol medium for induction of expression. The cells were disrupted and the intracellular protein was extracted. The results of ELISA and Western Blot showed that 4 strains of GS1 1 5 / HIS + MUT + expressed HBVM protein.