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目的 :为了探讨IL 12对狼疮肾炎PBMC发挥生物学作用的细胞内信号传递机制。方法 :采用MTT、免疫沉淀、免疫印迹等方法 ,检测IL 12对PBMC增殖及STAT3、STAT4的作用 ,并比较在狼疮肾炎活动期、静止期的差别。结果 :发现与正常人对照及静止期相比 ,IL 12对活动期PBMC不需要IL 2的预诱导 ,可促进PBMC增殖并在短时间内激活STAT3、STAT4,而IL 2对STAT4无作用。结论 :表明IL 12对PBMC的作用可通过STAT3、STAT4途径发挥 ,活动期PBMC存在异常活化 ,这可能是IL 12促进狼疮肾炎PBMC参与发病的细胞内机制之一
OBJECTIVE: To investigate the intracellular signal transduction mechanism of IL-12 on the biological effects of lupus nephritis PBMC. Methods: MTT, immunoprecipitation and Western blotting were used to detect the effect of IL-12 on PBMC proliferation and STAT3, STAT4, and to compare the differences in active and quiescent phases of lupus nephritis. Results: Compared with normal control and quiescent control, IL 12 did not require pre-induction of IL 2 in PBMCs, which could promote PBMC proliferation and activate STAT3 and STAT4 in a short time, while IL 2 had no effect on STAT4. CONCLUSION: The results suggest that the effect of IL 12 on PBMC may be through the STAT3 and STAT4 pathways, and the abnormal activation of PBMC in the active phase may be one of the intracellular mechanisms involved in the pathogenesis of IL-12 in promoting the pathogenesis of lupus nephritis.