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目的 观察低剂量辐射对儿童T -淋巴细胞DNA、蛋白质合成能力的影响。方法 通过体外细胞培养 ,用14 C -胸腺嘧啶脱氧核苷 (14 C -TdR)、3H -酪氨酸 (3H -Tyr)掺入法观察。 结果 在 5及 10cGy低剂量辐射下14 C -TdR、3H -Tyr掺入均有显著增加。婴幼儿组在 5cGy辐射下增加最为显著。学龄前及学龄儿童则在 10cGy辐射下增加最为显著。营养不良患儿与婴幼儿组相比较在自然状态下14 C -TdR掺入明显下降 ,不同低剂量辐射作用趋势相似 ,但强度偏低。结论 适宜的低剂量辐射可刺激儿童T -淋巴细胞DNA、蛋白质合成。对营养不良患儿的刺激效应低于正常儿童
Objective To observe the effects of low dose radiation on T - lymphocyte DNA and protein synthesis in children. Methods In vitro cell culture was observed with 14 C - thymidine incorporation and 3H - Tyr incorporation. Results The incorporation of 14 C -TdR and 3H-Tyr significantly increased at low doses of 5 and 10 cGy doses. The infant group increased most significantly at 5 cGy radiation. Preschool and school-age children increased most significantly at 10 cGy radiation. Compared with infants and young children, malnourished infants had significantly decreased incorporation of 14 C -TdR under natural conditions. The effects of different low-dose radiation were similar, but the intensity was lower. Conclusion Suitable low dose radiation can stimulate T - lymphocyte DNA and protein synthesis in children. Stimulation effect on children with malnutrition is lower than normal children