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目的采用HPLC测定犬血浆中的头孢他美钠。方法采用高氯酸直接沉淀血浆蛋白,离心后取上清液进样测定。采用Hypersil BDS C18色谱柱(200 mm×4.6 mm,5μm),流动相为甲醇-5 mmol.L-1高氯酸(24:76,pH2.62),流速1.0 mL.min-1,柱温35℃,检测波长265 nm。结果头孢他美钠0.6032~603.2475μg.mL-1与峰面积的线性关系良好(r=0.9998),低、中、高浓度的方法回收率分别为96.65%、87.41%、95.28%,日内RSD分别为1.25%、7.07%、4.61%(n=5),日间RSD分别为6.33%、10.26%、1.88%(n=5)。结论所建方法简便、灵敏准确,可为头孢他美钠及相关制剂的体内研究提供参考。
Objective To determine ceftazidime in dog plasma by HPLC. Methods Perchloric acid was used to directly precipitate plasma protein and centrifuged to determine the supernatant. The mobile phase was methanol-5 mmol.L-1 perchloric acid (24:76, pH2.62) at a flow rate of 1.0 mL · min-1 on a Hypersil BDS C18 column (200 mm × 4.6 mm, 5 μm) 35 ℃, detection wavelength 265 nm. Results The linear relationship between ceftazidime sodium and peak area was 0.6032 ~ 603.2475μg.mL-1 (r = 0.9998), and the recoveries were 96.65%, 87.41% and 95.28% Were 1.25%, 7.07%, 4.61% (n = 5). The daytime RSD were 6.33%, 10.26%, 1.88% (n = 5) respectively. Conclusion The proposed method is simple, sensitive and accurate, which can provide reference for the in vivo study of ceftazime sodium and its related preparations.