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目的通过检测急性心肌梗死患者及对照人员的血小板膜糖蛋白Ia-807基因多态性,探讨该多态性与急性心肌梗死发病的关系。方法血小板膜糖蛋白Ia基因-807多态性分析:①碘化钾法提取人基因组DNA;②多聚酶链反应(PCR)扩增目的基因片段;③酶切产物经2%琼脂糖凝胶电泳分离后,紫外灯下检测酶切结果。SPSS11.5软件分析数据。结果①急性心肌梗死组血小板膜糖蛋白Ia807TT+TC基因型携带者频率显著高于对照组。②急性心肌梗死患者平均甘油三脂水平显著高于对照组。结论①血小板膜糖蛋白Ia807T等位基因可能是急性心肌梗死发病的独立遗传性危险因素。②急性心肌梗死发病与血清甘油三脂水平有关。
Objective To detect the polymorphism of platelet membrane glycoprotein Ia-807 in patients with acute myocardial infarction and control subjects and to explore the relationship between the polymorphism and the incidence of acute myocardial infarction. Methods The platelet membrane glycoprotein Ia gene -807 polymorphism analysis: ① potassium iodide extraction of human genomic DNA; ② polymerase chain reaction (PCR) to amplify the target gene fragment; ③ enzyme digestion products by 2% agarose gel electrophoresis separation, UV detection of the results of enzyme digestion. SPSS11.5 software to analyze the data. Results ① The frequency of platelet membrane glycoprotein Ia807TT + TC genotype in acute myocardial infarction group was significantly higher than that in control group. ② The average level of triglyceride in patients with acute myocardial infarction was significantly higher than that in the control group. Conclusion ① Platelet membrane glycoprotein Ia807T allele may be an independent risk factor for the development of acute myocardial infarction. ② The incidence of acute myocardial infarction and serum triglyceride levels.