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本文论述了在HPLC分析中用α′参数定性的可行性。由列举的实验数据及萤光谱图可见,免去测定t_0采用可以直观获得的绝对保留时间的比值α′进行定性的方法是简单、可靠的。
This article discusses the feasibility of using α ’parameters in HPLC analysis. As can be seen from the experimental data and the fluorescence spectra enumerated, it is simple and reliable to dispense with the determination of t_0 using the ratio α ’of the absolute retention time which can be directly obtained.