论文部分内容阅读
目的:体外分离和培养新生大鼠大脑皮质神经干细胞并进行鉴定,为用于脑梗死动物模型梗死区移植细胞作准备。方法:分离新生大鼠皮质神经干细胞,进行体外培养,使用免疫细胞荧光染色技术对细胞的特性进行鉴定,并对细胞的胆碱能特征进行鉴定。结果:获得了Nestin阳性的神经干细胞,其分化后可获得MAP 2、GFAP和CNPase阳性的神经元、星形胶质细胞和寡突胶质细胞;通过胆碱能鉴定发现大脑皮质干细胞可分化为胆碱能神经元。结论:体外分离和培养的大脑皮质神经干细胞具有增殖分化的能力,并可以分化为胆碱能神经元,有望应用于皮质损伤后认知障碍及运动障碍的细胞移植治疗。
OBJECTIVE: To isolate and culture neural stem cells of neonatal rat cerebral cortex in vitro and to identify them for the preparation of transplanted cells in the infarct zone of an animal model of cerebral infarction. Methods: Newborn rat cortical neural stem cells were isolated and cultured in vitro. The characteristics of cells were identified by immunofluorescence staining and the cholinergic characteristics of the cells were identified. RESULTS: Nestin-positive neural stem cells were obtained and differentiated to obtain MAP 2, GFAP and CNPase-positive neurons, astrocytes and oligodendrocytes. Cholinergic identification showed that cerebral cortex stem cells could differentiate into Cholinergic neurons. CONCLUSION: Neural stem cells cultured and isolated from cortex have the ability of proliferation and differentiation and can differentiate into cholinergic neurons. It is expected to be used in the treatment of cognitive impairment and dyskinesia after cortical injury.