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目的观察银杏叶提取物(extract of ginkgo bilobaleaf,EGB761)在高糖高胰岛素作用下对视网膜Müller细胞活性的改变及其保护作用。方法用30mmol/L葡萄糖和(或)200U/L的胰岛素处理大鼠视网膜Müller细胞48小时,分为高糖高胰岛素组:200U/L胰岛素+30mmol/L葡萄糖;高胰岛素组:200U/L胰岛素;对照组:普通低糖型DMEM培养(含葡萄糖5mmol/L)。提前0.5小时加入60μg/mL、120μg/mL的EGB761,观察对各组细胞的保护作用,48小时后用MTT法测定视网膜Müller细胞的活性。结果高糖高浓度胰岛素处理后Müller细胞活性降低,与对照组相比差异有统计学意义(P<0.05)。而60或120μg/mL的EGB761能上调高浓度葡萄糖和高胰岛素处理后的视网膜Müller细胞的活力,且在单纯高胰岛素组中更佳,与无EGB761处理组相比差异有统计学意义(P<0.05)。结论高浓度葡萄糖和高胰岛素在短期内可使视网膜Müller细胞的活性降低,一定浓度的EGB761可减轻高糖高胰岛素对视网膜Müller细胞的损伤。
Objective To observe the changes and protective effects of extract of ginkgo bilobaleaf (EGB761) on retinal Müller cells under high glucose and high insulin concentration. Methods Retinal Müller cells were treated with 30mmol / L glucose and / or 200U / L insulin for 48 hours and divided into high glucose and high insulin group: 200U / L insulin and 30mmol / L glucose; high insulin group: 200U / L insulin ; Control group: ordinary low glucose DMEM culture (containing glucose 5mmol / L). EGB761 (60μg / mL, 120μg / mL) was added 0.5h beforehand to observe the protective effect on each group of cells, and the activity of retinal Müller cells was measured by MTT method 48h later. Results The Müller cells activity decreased with high glucose and high concentration of insulin, which was significantly different from the control group (P <0.05). While EGB761 at 60 or 120 μg / mL increased the vitality of retinal Müller cells treated with high glucose and insulin, and it was better in simple hyperinsulinemia group than in EGB761-treated group (P < 0.05). Conclusion High concentrations of glucose and insulin can decrease the activity of retinal Müller cells in a short time. EGB761 can reduce the damage of retinal Müller cells induced by high glucose and high insulin.