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目的鉴定玉麦中可吸收入血的micro RNAs(mi RNAs)类活性物质,并分析其靶基因功能。方法采用二代高通量测序法,以正常小鼠血浆mi RNAs为空白对照,测定喂食玉麦匀浆液小鼠血浆中mi RNAs的总量,并与玉麦mi RNAs比对,鉴定吸收入体内的玉麦mi RNAs。采用Target Scan与Mi Randa软件,预测玉麦mi RNAs可能作用于人类m RNA靶基因,并通过Gene Ontology(GO)与Kyoto Encyclopedia of Genes and Genomes(KEGG)分析靶基因的相关功能和信号途径。结果共鉴定出12个可吸收入体内的玉麦mi RNAs。GO与KEGG分析显示这些mi RNAs可能调控的靶基因及其参与的生物学功能包括胰岛素信号通路、癌症通路、MAPK通路等相关信号通路,其中mi R4995、mi R156a、mi R396e为玉麦可能的主要核酸活性物质。结论 mi RNAs可能为玉麦中的一种新的活性物质。
Objective To identify microRNAs (miRNAs) active substances that can be absorbed into the blood of jade and to analyze the function of target genes. Methods The second generation of high-throughput sequencing method was used to measure the total amount of mi RNAs in the plasma of mice fed with the homogenate of jade and wheat miRNAs in normal mice. The miRNAs were compared with the miRNAs of jade wheat and identified to be absorbed into the body Of jade miRNAs. Target Scan and Mi Randa software were used to predict the possible roles of zea mi RNAs in human m RNA target genes and to analyze the related functions and signaling pathways of target genes by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). RESULTS: Twenty jade miRNAs that could be absorbed into the body were identified. GO and KEGG analysis showed that these miRNAs may regulate the target genes and their biological functions involved in insulin signaling pathway, cancer pathway, MAPK pathway and other related signaling pathways, mi R4995, mi R156a, mi R396e may be the main Nucleic acid active substance. Conclusion mi RNAs may be a new active substance in jade.