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目的:建立定量分析脾氨肽口服冻干粉中游离氨基酸与肽含量的方法。方法:采用氨基酸分析仪,以外标法定量测定游离氨基酸与酸水解后总氨基酸的含量,总氨基酸与游离氨基酸的差值即为肽的含量。结果:各氨基酸在各自测定浓度20%~180%的范围内线性关系均良好(r均在0.999以上);游离氨基酸回收率在97.2%~103.9%之间,总氨基酸的回收率在93.1%~101.7%之间,RSD均小于2.0%(n=9)。结论:本方法能够准确地测定样品中游离氨基酸及肽的含量。
OBJECTIVE: To establish a method for quantitative analysis of free amino acids and peptides in oral freeze-dried powder of spleen amino peptide. Methods: Amino acid analyzer was used to quantitatively determine the content of total amino acids after free amino acids and acid hydrolysis by external standard method. The difference between total amino acids and free amino acids was the content of peptides. Results: The linearity of each amino acid in the range of 20% -180% was good (r> 0.999). The recoveries of free amino acids ranged from 97.2% to 103.9%, and the total amino acid recoveries ranged from 93.1% 101.7%, RSD were less than 2.0% (n = 9). Conclusion: This method can accurately determine the content of free amino acids and peptides in the sample.