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研究了暗纹东方鲀排卵后授精时间与受精率的关系。对 3龄人工养殖的暗纹东方鲀采用多次注射外源激素的方法, 诱导其卵母细胞成熟并排卵。分别在不同时刻对排卵后的卵母细胞进行人工授精, 在原肠中期检查受精率。结果表明, 随着排卵后授精时间的延长, 受精率逐渐下降, 18h时受精率降为 0。排卵后 0、2、4h时授精的受精率, 分别为 83. 75%、78 .75%和 75. 5%, 无显著差异 (P>0 .05), 而其它不同授精时间之间的平均受精率均具有显著性差异 (P<0. 05)。对于不同时刻的平均受精率与排卵后授精时间两者关系进行拟合, 得出它们之间的关系为y= 0 .0352x3 -0 .9877x2 +1 .7976x+81. 953 (R2 =0 .996 ), 相关系数较高, 说明应用 3阶函数曲线能很好地表达两者之间的关系。
The relationship between fertilization and fertilization after ovulation was studied. On the third instar cultured Takifugu obscurus repeated injection of exogenous hormones, induction of oocyte maturation and ovulation. At different times of ovulation after oocyte in artificial insemination, metaphase check the fertilization rate. The results showed that with the fertilization time after ovulation, fertilization rate gradually decreased, fertilization rate dropped to 0 at 18h. The fertilization rates at 0, 2 and 4 h after ovulation were 83.75%, 78.75% and 75.5%, respectively, with no significant difference (P> 0.05), while the average of other insemination times Fertilization rates were significantly different (P <0. 05). The relationship between the average fertilization rate at different times and the time of fertilization after ovulation was fitted, and the relationship between them was y = 0 .0352x3 -0.9877x2 + 1.7976x + 81.95 (R2 = 0.996 ), The higher the correlation coefficient, indicating the application of third-order function curve can well express the relationship between the two.