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在本研究中,建立了高效、灵敏、准确的超快速液相色谱法同时快速分析测定有柄石韦中10个指标成分(新绿原酸、绿原酸、隐绿原酸、1-咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、紫云英苷、圣草酚-7-O-β-D-吡喃葡萄糖醛酸苷、山奈酚-3,7-双-O-葡萄糖苷)的含量。采用了Kromasil 100-2.5C18(100 mm×2.1 mm,2.5μm)C18快速色谱柱,以0.1%甲酸水和0.1%甲酸甲醇溶液为洗脱剂洗脱,检测波长为326 nm,流速为0.4 mL/min。10个成分在此色谱条件下能达到基线分离,标准曲线线性关系良好(r 2>0.9998),日内与日间精密度、重复性、稳定性均符合实验要求,加样回收率范围为99.1%–104.5%。对20批不同来源的有柄石韦样品进行了含量测定。此研究为有柄石韦中多成分含量测定提供了高效、灵敏、准确的快速分析方法。此外,还对20份样品进行了聚类分析和主成份分析,结果显示此方法可以很好地区分20份样品的来源及资源分布情况。
In this study, an efficient, sensitive and accurate ultra-fast liquid chromatographic method was developed for the simultaneous determination of 10 index components (chlorogenic acid, chlorogenic acid, chlorogenic acid, 1-caffeoyl Quinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, albinyl, eriodictyol-7-O- β-D-glucopyranoside, kaempferol-3,7-bis-O-glucoside). A C18 column with Kromasil 100-2.5C18 (100 mm × 2.1 mm, 2.5 μm) was used to elute with 0.1% formic acid and 0.1% formic acid in methanol. The detection wavelength was 326 nm and the flow rate was 0.4 mL / min. Under these chromatographic conditions, 10 components could be separated from the baseline, and the standard curve showed a good linearity (r 2> 0.9998). The precision, repeatability and stability of the 10 components were in line with the experimental requirements. The recoveries ranged from 99.1% -104.5%. Twenty batches of shalyse samples from different sources were assayed. This study provides a highly efficient, sensitive and accurate method for the rapid analysis of multi-component determination of Schistosoma japonicum. In addition, cluster analysis and principal component analysis were performed on 20 samples. The results show that this method can well distinguish the source and resource distribution of 20 samples.