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目的 探讨CD137分子在衰老小鼠脾脏T细胞表面表达的规律。方法 通过注射D 半乳糖建立亚急性衰老小鼠模型。分离衰老模型组小鼠及正常对照组小鼠的脾脏T细胞 ,经ConA刺激后进行培养 ,采用流式细胞术检查ConA刺激后不同培养时间的T细胞上CD137的表达 ,并进行比较。结果 小鼠脾脏T细胞经过ConA刺激后 ,正常对照组小鼠T细胞上CD137分子的表达高峰出现于刺激后第 6天 ,平均表达率为 48.5 % ,之后迅速下降。 1个月和2个月衰老模型组小鼠T细胞上CD137分子在表达高峰的表达率平均分别为 39.1%和 32 .8% ,均显著低于正常对照组小鼠 (P <0 .0 1) ,其中 1个月模型组小鼠T细胞上CD137分子表达高峰的出现时间及下降规律与正常对照组小鼠相同 ;而 2个月衰老模型组小鼠T细胞上CD137分子的表达高峰出现于刺激后第 4天 ,第 9天缓慢下降至与正常对照组相同时间的水平。结论 亚急性衰老小鼠T细胞上CD137分子的表达低于正常水平。至衰老过程的后期 ,T细胞上CD137分子的表达高峰提前出现 ,表达水平达到峰值后下降速度较正常小鼠缓慢 ,提示CD137分子在机体抗衰老过程中 ,具有调节T细胞功能的作用
Objective To investigate the expression of CD137 on the surface of T lymphocytes in the spleen of aging mice. Methods A sub-acute aging mouse model was established by injection of D-galactose. The splenic T cells of the mice in the aging model group and the normal control group were isolated and stimulated with ConA. The expression of CD137 on T cells stimulated with ConA was detected by flow cytometry and compared. Results After splenic T cells were stimulated with ConA, the expression of CD137 on T cells of normal control mice appeared on the 6th day after stimulation, and the average expression rate was 48.5%, then decreased rapidly. The peak expression rates of CD137 on T cells of mice in aging model at 1 month and 2 months were 39.1% and 32.8% respectively, which were significantly lower than those in normal mice (P <0.01) ), In which the appearance time and declination of CD137 on T cells in one month model group were the same as those in normal control group; while the expression peak of CD137 on T cells in two-month aging model group appeared in On the 4th day and the 9th day after stimulation, the levels of IL-6 and IL-6 decreased slowly to the same level as the normal control group. Conclusion The expression of CD137 on T cells of subacute aging mice is lower than the normal level. At the later stage of aging, the expression of CD137 on T cells peaked earlier than that of normal mice, indicating that CD137 molecules may play a regulatory role in the anti-aging process of T cells.