Leaching of nickel and cobalt from two physical grades(S1,125-190 μm,coarser and S3,53-75 μm,finer) of chromite overburden was achieved by treating the overburden(2% pulp density) with 21-d culture filtrate of an Aspergillus niger strain grown in sucrose medium.Metal dissolution increases with ore roasting at 600℃ and decreasing particle size due to the alteration of microstructural properties involving the conversion of goethite to hematite and the increase in surface area and porosity as evident from X-ray diffraction(XRD),thermogravimetry-differential thermal analysis(DTTGA),and field emission scanning electron microscopy(FESEM).About 65% Ni and 59% Co were recovered from the roasted S3 ore employing bioleaching against 26.87% Ni and 31.3% Co using an equivalent amount of synthetic oxalic acid under identical conditions.The results suggest that other fungal metabolites in the culture filtrate played a positive role in the bioleaching process,making it an efficient green approach in Ni and Co recovery from lateritic chromite overburden. Leaching of nickel and cobalt from two physical grades (S1, 125-190 μm, coarser and S3, 53-75 μm, finer) of chromite overburden was achieved by treating the overburden (2% pulp density) with 21-d culture filtrate of an Aspergillus niger strain grown in sucrose medium. Metal dissolution increases with ore roasting at 600 ° C and decreasing particle size due to the alteration of microstructural properties involving the conversion of goethite to hematite and the increase in surface area and porosity as evident from X-ray About 65% Ni and 59% Co were recovered from the roasted S3 ore employing bioleaching against 26.87% Ni and 31.3% Co using (XRD), thermogravimetry-differential thermal analysis (DTTGA), and field emission scanning electron microscopy (FESEM) an equivalent amount of synthetic oxalic acid under identical conditions. the results suggest that other fungal metabolites in the culture filtrate played a positive role in the bioleaching process, making it an efficient green approach in Ni and Co recovery from lateritic chromite overburden.