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目的为了进一步探讨高原脑水肿的发生机制及高原环境下脑内ＥＴ与ＮＯ的释放与脑水肿的关系。方法２００只小鼠分别置于兰州（１５１７ｍ）、格尔木（２８００ｍ）和昆仑山口（４７６７ｍ）的不同海拔梯度下和高海拔区（４７６７ｍ）的不同时间暴露下（１、３、５、７、９、１１、１３、１５ｄ），分别测定脑内ＥＴ和ＮＯ含量及脑湿干重、脑温干比值和脑含水量。结果在急进高原初期脑内的ＥＴ释放量明显增加，ＮＯ／ＥＴ比值变小。进入高海拔区后３ｄ陡然下降。ＮＯ的释放量在急进高原的前１０ｄ都呈明显增高趋势，ＮＯ／ＥＴ比值明显增大，进入高原１３ｄ后脑内ＮＯ释放量开始下降。随着海拔梯度升高脑含水量和脑湿于比值明显增加，小鼠在高海拔区暴露下，脑含水量和脑湿干比值则随着暴露时间延长而增高，两者均以进入高海拔区的第ｇｄ为显著。在高海拔区暴露１１ｄ后虽两者都略有下降，但仍明显高于低海拔区脑含水量和脑湿干比值。组织学观察表明，进入高海拔区的第７ｄ神经细胞肿胀变圆，胞浆内出现空泡变性。相关分析表明，ＥＴ与脑含水量在进入高海拔区的初期呈正相关系，随后呈负相关关系；而ＮＯ与脑含水量之间始终呈正相关关系，有显著的相关性。结论在高原急性低氧暴露下，脑内一定量的ＮＯ释?
Objective To further explore the pathogenesis of high altitude brain edema and the relationship between brain ET and NO release and brain edema under high altitude environment. Methods 200 mice were exposed to different altitudes of Lanzhou (1517m), Golmud (2800m) and Kunlun Pass (4767m) and exposure to high altitude (4767m) for 1, 3, 5, 7, , 11, 13, 15d). The contents of ET and NO in the brain and the wet and dry weight of the brain, the ratio of brain temperature and dryness, and the brain water content were measured respectively. Results In the early plateau, ET release increased significantly in the brain and the ratio of NO / ET decreased. Into the high-altitude area after a sharp decline in 3d. The release of NO significantly increased in the first 10 days, and the NO / ET ratio increased significantly. The amount of NO released into the brain began to decrease after 13 days of entering the plateau. With the increase of altitude gradient, the ratio of brain water content and brain dampness increased significantly. When the mice were exposed in the high altitude area, the brain water content and brain wet and dry ratio increased with the extension of exposure time. District gd is significant. Although both of them decreased slightly after exposure for 11 days in high altitude area, they were still significantly higher than those in low altitude area. Histological observation showed that on the 7th day after entering the high altitude area, the swelling of the nerve cells became round, and vacuolar degeneration appeared in the cytoplasm. Correlation analysis showed that there was a positive correlation between ET and brain water content in the initial stage of high altitude area, followed by a negative correlation between ET and brain water content. There was a positive correlation between ET and brain water content, with significant correlation. Conclusion Acute hypoxia exposure in the plateau, a certain amount of brain NO release?