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用染色体组原位杂交技术对含有I■y■ws ■ltiolis Tzvelev、Thi■pyr■essar(?)icum Love染色体的普通小麦品系染色体切片进行了外源染色质鉴定,并对含有Hordeum chilense Rvem和Shll.、H.vulgare L.与Se■le oereuleL.异源种质染色体群进行了分析。以导入外源染色质的全套染色体组DNA为探针,与来自小麦的过量未标记的块植DNA进行DNA原位杂交。其方法是将外源染色质标记成黄绿色,而小麦染色体则表现为DNA复杂的桔红色荧光。细胞核选自幼苗根尖和药壁组织.鉴定外源染色体和染色体臂的染色体组探测方法.可以在细胞核分裂周期的所有时期进行计数,在前期和间期,二体系的大多数细胞核中,可以看到两个标记区,而单体系仅有一个标记区。在中期,可以直接看到异源染色体或其片段的形态,从而鉴定出异源染色质与小麦染色体的关系。
Chromosome sections of common wheat lines containing I ■ y ■ ws ■ ltiolis Tzvelev, Thi ■ pyr ■ essar (?) Icum Love chromosomes were identified by exogenous chromatin using chromosome-based in situ hybridization and hybridization was performed on chromosomes containing Hordeum chilense Rvem and Shll., H. vulgare L. and Se leolereule L. Heterologous germplasm populations were analyzed. The whole set of genomic DNA introduced into exogenous chromatin was used as a probe to perform DNA in situ hybridization with an excess of unlabeled plant-derived DNA from wheat. The method is to mark the exogenous chromatin yellow-green, while the wheat chromosomes display DNA complex orange-red fluorescence. The nuclei are selected from the root tips of the seedlings and the wall of the drug wall, and the method of detecting the chromosomes of exogenous chromosomes and chromosome arms can be counted at all stages of the mitotic cycle. In the early stage and the interphase, most nuclei of the two systems There are two marker zones, while the one system has only one marker zone. In the medium term, the morphology of heterologous chromosomes or their fragments can be seen directly to identify the relationship between heterologous chromatin and wheat chromosomes.