论文部分内容阅读
目的 动态观察 1,2 5-二羟基维生素 D3[1,2 5- ( OH) 2 D3]对 NH小鼠骨髓细胞培养在体外形成破骨细胞及其骨吸收的剂量效应和作用时象。方法 收集 NH小鼠骨髓细胞于含 10 %胎牛血清的α- MEM培养基中行体外培养 ,设置不同的 1,2 5- ( OH) 2 D3浓度组和给药时间组 ,并于培养第 3、6、9、12天观察记录抗酒石酸酸性磷酸酶 ( tartrate- resistant acid phosphatase,TRAP)阳性多核巨细胞 [或破骨细胞样细胞 ( osteroblast- like cell,OL C) ]以及骨磨片上骨吸收陷窝的数目。结果 高于 10 -9mmol/L的1,2 5- ( OH) 2 D3单一刺激可于培养第 6天诱导 TRAP阳性 OLC形成 ,并且可在骨磨片上观察到骨吸收陷窝 ;随 1,2 5- ( OH) 2 D3浓度的增高 ,OLC和骨吸收陷窝数目均随之增加 ;各浓度组 OLC数目于培养第9天达最高值 ,随后则趋于减少 ,而骨吸收陷窝数于培养第 9和第 12天均有所增加 ;采用单一 1,2 5-( OH) 2 D3浓度 ( 10 -8mmol/L) ,TRAP阳性 OL C和骨吸收陷窝见于培养 3d后用药和全程用药组 ,而仅在培养前 3d用药则不能诱导 OL C和骨吸收陷窝的形成。结论 1,2 5- ( OH) 2 D3可在体外诱导骨髓单核细胞分化形成 OLC并使其具有破骨活性 ,诱导作用的强弱与 1,2 5- ( OH) 2 D3的浓度相关 ,而且其作用时象可能是在培?
Objective To observe the dose-effect and time-effect of 1,2-dihydroxyvitamin D3 [1,2 5- (OH) 2 D3] on the formation of osteoclasts and their bone resorption in cultured NH mice bone marrow cells. Methods NH mouse bone marrow cells were collected and cultured in α-MEM containing 10% fetal bovine serum. Different concentrations of 1,2,5- (OH) 2 D3 and administration time groups were set up. At 6, 9, and 12 days, tartrate-resistant acid phosphatase (TRAP) -positive multinucleated giant cells [or osteoblast-like cells (OLC) The number of lacunae. Results A single stimulation of 1,2 5- (OH) 2 D3 above 10 -9 mmol / L induced TRAP-positive OLC formation on day 6 of culture and bone resorption lacuna was observed on the bone fragment; The number of OLC and bone resorption lacuna increased with the increasing of 5- (OH) 2 D3 concentration. The number of OLC in each concentration group reached the highest value on the 9th day of culture, then decreased, while the number of resorption lacuna Both the TRAP-positive OLC and the bone resorption lacuna were found after 3 days of culture and the whole course of treatment was treated with single 1,2 5- (OH) 2 D3 (10 -8 mmol / L) Group, whereas medication alone did not induce the formation of OLC and bone resorption lacuna only three days prior to culture. Conclusions 1,25 (OH) 2 D3 can induce the differentiation and differentiation of bone marrow mononuclear cells into osteoclasts and osteoclasts in vitro. The intensity of induction is related to the concentration of 1,2,5- (OH) 2 D3, And its role may be like training?