以高致瘤性K5 6 2e细胞和表达DAAO基因的KDfGC细胞建立裸鼠移植瘤模型 ,D 丙氨酸 (D Ala)腹腔注射 ,持续 3周 ,观察D Ala对移植瘤的治疗作用以及肿瘤、裸鼠重要脏器的病理变化。结果显示 ,经D Ala治疗 ,转基因肿瘤抑制率为 5 3 8% ,未转基因肿瘤抑制率为 0 3%。治疗组KDfGC细胞瘤体出现围绕血管分布的坏死。心、肝、肾治疗组与对照组均无明显病理改变。说明在体内DAAO+的K5 6 2e能被D Ala有效杀伤 ,并对重要脏器无明显损伤 The model of xenografted nude mice was established with high tumorigenic K5 6 2e cells and KDfGC cells expressing DAAO gene. D-Ala was injected intraperitoneally for 3 weeks to observe the therapeutic effect of D Ala on tumor xenografts, Pathological changes of vital organs in nude mice. The results showed that after D Ala treatment, the inhibition rate of transgenic tumor was 53.8%, and the inhibition rate of non-transgenic tumor was 0.3%. Treatment group KDfGC tumor appeared around the vascular necrosis. Heart, liver, kidney treatment group and control group had no significant pathological changes. Description of DAAO + K5 6 2e can be effectively killed by D Ala and no significant damage to vital organs