论文部分内容阅读
目的 探讨正常人胚胎胰岛在 Ｉ Ｌ１β、 Ｔ Ｎ Ｆα、 Ｉ Ｆ Ｎγ作用下， Ｉ Ｌ６ 的分泌及基因表达状况。方法 采用常规消化方法分离人胚胎胰岛并进行培养，分别加入ｒｈ Ｉ Ｌ１β、ｒｈ Ｔ Ｎ Ｆα、ｒｈ Ｉ Ｆ Ｎγ或同时加入２ 种或３ 种细胞因子，作用２４ 、４８ 小时收上清，进行 Ｉ Ｌ６ 、胰岛素、胰高血糖素活性检测和 Ｉ Ｌ６单抗中和实验，同时对３ 种细胞因子联合刺激的胰岛 Ｒ Ｎ Ａ 进行 Ｉ Ｌ６ ｍ Ｒ Ｎ Ａ 斑点杂交。结果 ３ 种细胞因子单独或联合刺激均可大幅度提高 Ｉ Ｌ６ 和胰高血糖素的分泌，同时不同程度地抑制胰岛素的分泌，细胞因子刺激胰岛的时间和强度与 Ｉ Ｌ６ 、胰岛素、胰高血糖素的分泌有明显的关系。斑点杂交结果显示３ 种细胞因子联合刺激的胰岛 Ｒ Ｎ Ａ 中 Ｉ Ｌ６ ｍ Ｒ Ｎ Ａ 含量明显高于对照组。结论 炎性细胞因子调节胰岛分泌 Ｉ Ｌ６ 和 Ｉ Ｌ６ 基因表达。
Objective To investigate the secretion of I L6 and its gene expression under the action of I L1β, T N Fα and I F Nγ in normal human embryonic islets. Methods Human embryonic islets were isolated and cultured by routine digestion method. Two or three kinds of cytokines were added into rh I L1β, rh T N Fα and rh I F Nγ, respectively. After 24 and 48 hours, I L 6, insulin, glucagon activity test and IL6 mAb neutralization experiments, while three kinds of cytokines jointly stimulate the islet R N A I L 6 m R N A dot hybridization. The results of three kinds of cytokines, alone or in combination, can significantly enhance the secretion of IL-6 and glucagon, while inhibiting insulin secretion to varying degrees, the time and intensity of cytokine-stimulated islet with I L 6, insulin , Glucagon secretion of a clear relationship. The result of dot blot showed that the content of I L6 m R N A in pancreatic islets stimulated by 3 cytokines was significantly higher than that of the control group. Conclusion Inflammatory cytokines regulate the expression of I L6 and I L6 genes in islets.