论文部分内容阅读
目的探讨局灶性脑缺血后脑组织巢蛋白(Nestin)的表达以及人参皂甙Rg1对其表达的影响。方法采用改良的线栓法制备大鼠大脑中动脉阻塞脑缺血模型,应用免疫组织化学技术检测脑缺血以及预先给予人参皂甙Rg1后不同时间段(1、3、7、14d)脑组织Nestin的表达水平。结果给予人参皂甙Rg1后大鼠脑组织Nestin标记细胞的数目及阳性部位明显增多。结论人参皂甙Rg1可使大鼠脑缺血后脑组织Nestin表达水平上调,提示人参皂甙Rg1可刺激脑组织神经干细胞的增殖及分化,这也可能是其发挥脑保护及抗衰老作用的机制之一。
Objective To investigate the expression of nestin in brain after focal cerebral ischemia and the effect of ginsenoside Rg1 on its expression. Methods The cerebral ischemic model of middle cerebral artery occlusion was established by a modified suture method. The brain tissue was detected by immunohistochemical technique and the pre-administration of ginsenoside Rg1 at different time points (1,3,7,14d). The level of expression. Results The number and positive sites of Nestin marker cells in rat brain tissue were significantly increased after ginsenoside Rg1 administration. Conclusion Ginsenoside Rg1 can upregulate the expression of Nestin in brain tissue after cerebral ischemia in rats, suggesting that ginsenoside Rg1 can stimulate the proliferation and differentiation of neural stem cells in brain tissue, which may also be one of the mechanisms that play a role in brain protection and anti-aging.