论文部分内容阅读
将分子标记辅助选择(MAS)和一个略作修改的轮回选择育种计划结合起来,目的将来自一个春性、稳定和纯黄DH品系No.2127-17的黄籽基因转育到一个半冬性的波里马恢复系恢5148-2中,以选育黄籽波里马恢复系。在本育种计划中,首先应用142条RAPD引物扫描12个优良的黄籽DH系,获得2个与恢5148-2背景恢复率最高的DH系。此后应用显性SCAR标记SCS1130和共显性标记SCA1分别分析BC1F1和BC2F1分离群体,选择黄籽的单株。为了更快的将所选单株的遗传背景恢复到恢5148-2,在BC1F1和BC2F1分离群体中分别应用88条RAPD和60对AFLP引物进行遗传背景分析,为了节约成本和简化分析,应用两步法进行此分析,逐步缩小分析群体样本,每次选择与恢5148-2遗传距离最小的3个单株作进一步的分析。最终,经共显性标记SCA1分析,从9个纯合黄籽单株中选择5株优良的恢复系作下一步分析,背景分析表明它们与恢5148-2的遗传距离小,为0.0157~0.0364,遗传背景得到较好的恢复。
Molecular marker-assisted selection (MAS) was combined with a slightly modified cycle-selective breeding program for the purpose of transferring the yellow-seeded gene from a spring, stable and pure yellow DH strain No.2127-17 to a semi-winter The Polima Restoration Department was restored to 5148-2 in order to bred the Yellow Seed Polima Restoration Department. In this breeding program, we first applied 142 RAPD primers to scan 12 excellent yellow-seed DH lines and obtained 2 DH lines with the highest background recovery rate of restorer 5148-2. After that, SCI marker SCS1130 and co-dominant marker SCA1 were used to analyze BC1F1 and BC2F1 segregation populations respectively and select the individual plants of yellow seed. In order to restore the genetic background of selected plants to restore 5148-2 faster, 88 RAPD and 60 AFLP primer pairs were used for genetic background analysis in the BC1F1 and BC2F1 segregating populations. In order to save costs and simplify the analysis, two This step by step analysis, and gradually reduce the analysis of population samples, each selection and restore 5148-2 genetic distance between the three smallest for further analysis. Finally, five dominant restorer lines from nine homozygous yellow seed plants were selected for further analysis by co-dominant marker SCA1 analysis. The background analysis showed that the genetic distance between them and Restorer5148-2 was small, ranging from 0.0157 to 0.0364 , The genetic background has been better recovery.