论文部分内容阅读
目的 探讨胰升糖素受体 (GCG- R)基因在中国人 2型糖尿病遗传背景中的地位。方法 运用聚合酶链反应-变性梯度凝胶电泳 (PCR- DGGE)技术对 2 0 0例无亲缘关系之中国云南昆明地区汉族人 (2型糖尿病患者 12 0例 ,健康对照者 80例 )的 GCG- R基因编码区进行了分子扫查。结果 仅发现 2例 2型糖尿病患者及 1例健康对照者的 GCG- R基因第 7外显子存在多态变异带型 ,这一杂合子多态变异基因型在 2型糖尿病组和对照组中的发生频率分别为 0 .0 17和0 .0 13,两者之间无显著性差异。此外 ,研究对象 GCG- R基因其它编码区的 PCR- DGGE扫查均未发现异常泳动片段。结论 GCG- R基因可能不是中国昆明地区汉族人 2型糖尿病的主要致病基因。
Objective To investigate the role of glucagon receptor (GCG-R) gene in the genetic background of type 2 diabetes in Chinese. Methods PCR-DGGE was used to detect the GCG of 200 Han unrelated individuals in Kunming, Yunnan Province, China (120 cases of type 2 diabetes mellitus and 80 healthy controls) by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) - R gene coding region of the molecular scanning. Results Only polymorphism of GCG-R gene exon 7 was found in 2 patients with type 2 diabetes mellitus and 1 healthy control. The heterozygous heterozygous variant genotypes of type 2 diabetes mellitus and control group The frequency of occurrence was 0 .0 17 and 0 .0 13, no significant difference between the two. In addition, none of the PCR-DGGE scans of the other coding regions of the GCG-R gene of the study subjects found abnormal motile fragments. Conclusion GCG-R gene may not be the main pathogenic gene of type 2 diabetes in Han nationality in Kunming, China.