二氧化硫对实验性腹主动脉-下腔静脉分流大鼠肺动脉压力的影响

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目的研究二氧化硫(SO2)对高肺血流性肺动脉高压的调节作用。方法 25只雄性Sprague Dawley大鼠随机分为对照组(n=8)、分流组(n=8)和分流+SO2供体组(n=9)。对分流组和分流+SO2供体组大鼠行腹主动脉-下腔静脉分流术;分流+SO2供体组大鼠每天腹腔内注射亚硫酸钠/亚硫酸氢钠(Na2SO3/NaHSO3)85 mg·kg-1·d-1,8周后以右心导管的方法测定3组大鼠肺动脉收缩压(PASP)、肺动脉平均压(PAMP)及肺动脉舒张压(PADP)。结果分流组大鼠PASP显著高于对照组大鼠[(3.91±0.66)kPa vs(2.26±0.47)kPa,P<0.05];分流+SO2供体组大鼠PASP显著低于分流组大鼠[(3.04±0.36)kPa vs(3.91±0.66)kPa,P<0.05];分流+SO2供体组大鼠PASP较对照组大鼠[(3.04±0.36)kPa vs(2.26±0.47)kPa,P<0.05]显著增高。分流组大鼠PADP较对照组大鼠显著升高[(1.81±0.45)kPa vs(1.26±0.32)kPa,P<0.05];而分流+SO2供体组大鼠PADP与分流组大鼠比较差异无统计学意义[(1.49±0.19)kPa vs(1.81±0.45)kPa,P>0.05]。分流组大鼠PAMP显著高于对照组大鼠[(2.53±0.43)kPa vs(1.60±0.36)kPa,P<0.05];分流+SO2供体组大鼠PAMP显著低于分流组大鼠[(2.01±0.23)kPa vs(2.53±0.43)kPa,P<0.05]。分流+SO2组PAMP显著高于对照组[(2.01±0.23)kPa vs(1.60±0.36)kPa,P<0.05]。结论 SO2可明显降低高肺血流大鼠的PASP和PAMP。 Objective To study the regulatory effect of sulfur dioxide (SO2) on pulmonary hypertension with pulmonary hypertension. Methods Twenty - five male Sprague Dawley rats were randomly divided into control group (n = 8), shunt group (n = 8) and shunt + SO2 donor group (n = 9). The abdominal aorta-inferior vena cava was shunted in the shunt group and the shunt + SO2 donor group. The rats in shunt + SO2 donor group were intraperitoneally injected sodium sulphite / sodium bisulfite (Na2SO3 / NaHSO3) 85 mg · kg The pulmonary artery systolic pressure (PASP), pulmonary artery mean pressure (PAMP) and pulmonary artery diastolic pressure (PADP) were measured by right heart catheterization in rats in each group. Results PASP in shunt group was significantly higher than that in control group [(3.91 ± 0.66) kPa vs (2.26 ± 0.47) kPa, P <0.05]. PASP in shunt + SO2 donor group was significantly lower than that in shunt group [ (3.04 ± 0.36) kPa vs (3.91 ± 0.66) kPa, P <0.05]. PASP in shunt + SO2 donor group was significantly lower than that in control group [(3.04 ± 0.36) kPa vs (2.26 ± 0.47) kPa, P < 0.05] was significantly higher. PADP in shunt group was significantly higher than that in control group [(1.81 ± 0.45) kPa vs (1.26 ± 0.32) kPa, P <0.05], while there was difference between PADP and shunt group in shunt + SO2 donor group No statistical significance [(1.49 ± 0.19) kPa vs (1.81 ± 0.45) kPa, P> 0.05]. PAMP in shunt group was significantly higher than that in control group [(2.53 ± 0.43) kPa vs (1.60 ± 0.36) kPa, P <0.05]; PAMP in shunt + SO2 donor group was significantly lower than that in shunt group [ 2.01 ± 0.23) kPa vs (2.53 ± 0.43) kPa, P <0.05]. PAMP in shunt + SO2 group was significantly higher than that in control group [(2.01 ± 0.23) kPa vs (1.60 ± 0.36) kPa, P <0.05]. Conclusion SO2 can significantly reduce the PASP and PAMP in high pulmonary blood flow rats.
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