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目的制备能特异性识别莱克多巴胺(ractopamine,RCT)的分子印迹聚合物(molecularly imprinted polymers,MIPs),将该印迹聚合物与微流控化学发光法结合起来,以制备高选择性的化学发光传感器。方法利用沉淀聚合法制备莱克多巴胺印迹聚合物,采用扫描电镜、红外光谱法、吸附实验对其进行表征;以该印记聚合物为识别元件,以微流控芯片为流通反应池,并以化学发光仪作为检测器,通过流动注射分析法来检测猪肝和牛肉中的莱克多巴胺。结果在最佳条件下,测得莱克多巴胺线性范围6～960 ng/mL,线性回归方程为?I=4.8777C-9.7851,r=0.9907,检出限为0.83 ng/mL,对10 ng/mL莱克多巴胺平行测定11次,其RSD(相对标准偏差)为7.1%。应用该方法成功分析了猪肝和牛肉中RCT含量,回收率可达90.3%~99.8%。结论本方法快速、准确、样品前处理简单,能很好地用于食品中莱克多巴胺残留的检测。
Objective To prepare molecularly imprinted polymers (MIPs) that can specifically recognize ractopamine (RCT). The imprinted polymers were combined with microfluidic chemiluminescence to prepare highly selective chemiluminescence sensors . Methods Raman dopamine imprinted polymers were prepared by precipitation polymerization. The polymers were characterized by scanning electron microscopy, infrared spectroscopy and adsorption experiments. The imprinted polymer was used as the identification element and the microfluidic chip was used as the flow-through reaction chamber. The chemiluminescence The instrument serves as a detector for the detection of ractopamine in pork liver and liver by flow injection analysis. Results Under the optimal conditions, the linear range of ractopamine was 6 ~ 960 ng / mL, the linear regression equation was I I = 4.8777C-9.7851, r = 0.9907 with a detection limit of 0.83 ng / mL. Ractopamine was tested 11 times in parallel with a RSD (relative standard deviation) of 7.1%. Using this method, the RCT content in pork liver and liver was successfully analyzed, the recovery rate was 90.3% ~ 99.8%. Conclusion The method is rapid, accurate and simple in sample preparation. It can be used to detect ractopamine residues in foodstuffs.