论文部分内容阅读
The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricta(GenBank accession number EU880278) has been determined and is reported here.We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing.Contained within the 17 225 base pairs(bp) are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes.The S.constricta mitochondrial DNA(mtDNA) did not contain a gene for atp8,similar to the mtDNA of Crassostrea virginica,Crassostrea giga and Mytilus edulis.The S.constricta mtDNA is 67.0% A+T(A 25.9%,C 10.5%,G 22.5%,and T 41.1%).This value is higher than that for many invertebrate mitochondrial genomes.Only 19 putative tRNA genes are present in S.constricta and 27 noncoding regions,of which two are large in size.The trnE and trnW genes as well as a second trnS were absent in S.constricta.The gene arrangement of S.constricta is different from the other Bivalvia genomes.
The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricta (GenBank accession number EU880278) has been determined and is reported here. We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing. Contained within the 17 225 base pairs (bp) are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochondrial DNA (mtDNA) did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica, Crassostrea giga and Mytilus edulis.The S. constricta mtDNA is 67.0% A + T (A 25.9%, C 10.5%, G 22.5%, and T 41.1%). This value is higher than that for many invertebrate mitochondrial genomes. Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which two are large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta. The gene arrangement of S. constricta is different from the other Bivalvia ge nomes.