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目的探讨染锰鼠支持细胞波形蛋白(vimentin,VM)表达与精子数量关系。方法雄性SD大鼠48只随机分为6组,15,30 mg/kg MnCl2组分别染锰4周和6周,空白对照组给予等溶生理盐水(4,6),腹腔注射;取睾丸和附睾分别检测支持细胞VM表达(免疫组织化学法)和精子数量。结果染锰15,30 mg/kg 4和6周组支持细胞波形蛋白阳性细胞率分别为(30.55±2.33)%,(9.01±2.27)%和(7.62±1.08)%,(2.33±0.63)%;精子数量分别为(0.53±0.11),(0.47±0.06)和(0.26±0.07),(0.23±0.04)×107/mL;与空白对照组比较,各染锰组支持细胞VM阳性细胞率及精子数量均明显降低(P<0.01),并呈一定时间-效应和剂量-效应关系;各组大鼠精子数量和支持细胞VM阳性细胞率呈正相关(r=0.895,P<0.01)。结论过量锰可诱发支持细胞VM表达降低,导致大鼠精子数量减少,可能是大鼠生精障碍的主要分子机制之一。
Objective To investigate the relationship between the expression of vimentin (VM) and sperm count in manganese-bearing rats. Methods Forty-eight male Sprague-Dawley rats were randomly divided into 6 groups. Manganese supplemented with 15 and 30 mg / kg MnCl2 for 4 weeks and 6 weeks, respectively. The blank control group was given isotonic saline (4, 6) Epididymis were used to detect the expression of supporting cells VM (immunohistochemical method) and sperm count. Results The percentage of vimentin-positive cells in the groups receiving 30 and 45 mg / kg manganese for 4 and 6 weeks were (30.55 ± 2.33)%, (9.01 ± 2.27)% and (7.62 ± 1.08)%, (2.33 ± 0.63)%, ; The numbers of sperm were (0.53 ± 0.11), (0.47 ± 0.06) and (0.26 ± 0.07) and (0.23 ± 0.04) × 107 / mL, respectively. Compared with the blank control group, The number of sperm in each group was positively correlated with the number of VM-positive cells (r = 0.895, P <0.01). Conclusion Excessive Mn can reduce the expression of VM in supportive cells, resulting in the decrease of sperm count in rats, which may be one of the major molecular mechanisms in rats with dyszoospermia.