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目的探讨小儿急性淋巴细胞白血病（ＡＬＬ）的克隆多样性。方法：聚合酶链反应扩增小儿ＡＬＬ的免疫球蛋白重链（ＩｇＨ）和Ｔ细胞受体（ＴＣＲγ）重排基因，用单链构象多态性（ＳＳＧＰ）、以及异源双链形成（ＨＤＦ）方法，分析基因重排方式。结果：３７例中，单克隆的ＩｇＨ／ＴＣＲ，基因重排中除了单等位基因重排１１例（３３％）外，有１６例（４９％）是双等位基因重排，另６例是寡克隆重排。结论：用抗原受体重排基因分析可以发现，小儿ＡＬＬ存在克隆多样性现象，且初步提示预后较差。
Objective To investigate the clonal diversity of pediatric acute lymphoblastic leukemia (ALL). Methods: Polymerase chain reaction (PCR) was used to amplify the immunoglobulin heavy chain (IgH) and T cell receptor (TCRγ) rearrangement genes in children with ALL. Single strand conformation polymorphism (SSGP) and heteroduplex formation ) Method, analysis of gene rearrangement. Results: Of the 37 cases, 16 cases (49%) had dual allele rearrangement in addition to 11 cases (33%) of single allele rearrangements in IgH / TCR and 6 cases Is oligoclonal rearrangement. Conclusion: Using gene rearrangement analysis of antigen receptor, we found that there is a phenomenon of clonal diversity in pediatric ALL, and initially suggests poor prognosis.